Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function.
Autophagy, a process of bulk protein degradation through an autophagosomic-lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells.
Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer.
Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons.
In gene transfer studies, beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication.
Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells.
Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections.
Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses.
Applications:Suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry (paraffin).
Other applications not tested.
Recommended Dilution:Western Blot: 1:2000-1:5000, a band is seen at ~52 kDImmunofluorescence: mouse tissueOptimal dilution determined by the researcher.
Storage and Stability:Store at 2-8°C.
Do Not Freeze! Aliquots are stable for at least 3 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS. Labeled with HRP. No preservative added.
Specificity：Recognizes human Beclin 1. 94% identity with the mouse sequence and 88% identity with the rat sequence.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Internal synthetic peptide to human Beclin 1, containing residues within 1-100. Cellular Localization: Cell Membrane, Cytoplasmic and Golgi Apparatus. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
