Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function.
Autophagy, a process of bulk protein degradation through an autophagosomic-lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells.
Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer.
Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons.
In gene transfer studies, beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication.
Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells.
Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections.
Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses.
Applications:Suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry (paraffin) and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2000-1:5000, a band is seen at ~52 kDImmunofluorescence: mouse tissueOptimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 3 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS. Labeled with Biotin.
Specificity：Recognizes human Beclin-1 at ~52kD. Species Sequence Homology: Mouse (94%), rat (88%)
Isotype：IgG
Calc Applications Abbrev：IF IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to within aa1-100 from human Beclin-1. Cellular Localization: Cell membrane, cytoplasmic, golgi apparatus. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
