Bcl-x, a member of the Bcl-2 protein family, inhibits cell death, or apoptosis (1).
Bcl-x is expressed as two isomeric forms, Bcl-xL and Bcl-xS, and is typically present in the cytosol in association with the mitochondrial membrane.
Bcl-xL forms heterodimers with various proteins, including Bax, Bak and Bcl-2 (2).
It has been found that heterodimerization with Bax does not seem to be required for anti-apoptotic activity (3).
Since Bcl-xL can form an ion channel in synthetic lipid membranes, there is a strong possibility that this property plays a role in heterodimerization-independent cell survival (4).
The Bcl-X(S) isoform promotes apoptosis.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot 1:10000, a band is seen ~26 kDOptimal dilution determined by the researcher.
Positive Controls:A431 Whole Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 3 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris-citrate/phosphate, pH 7-8, 0.09% sodium azide.
Specificity：Recognizes human BclX. Species homology: 100% hamster Bcl-X(L), canine Bcl-X(L), sheep Bcl-X(L), porcine Bcl-X(L), feline Bcl-X(L), 100% rabbit Bcl-X, rat Bcl-X, mouse Bcl-XThere are three known isoforms of Bcl-X; Bcl-X(S), Bcl-X(beta), and Bcl-X(L). The immunogen used to create this antibody has 100% homology to all three isoforms.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide made to an internal portion of the human protein (within residues 1-50). Mitochondrion membrane; Single-pass membrane protein (By similarity). Nucleus membrane; Single-pass membrane protein; Cytoplasmic side (By similarity).
