ATP-binding cassette (ABC) transporter genes are involved in the regulation of the amount of dietary cholesterol retained in the body.
The ABC transporter ABCG1 (ABC8) is expressed on the cell surface and in intracellular compartments of cholesterol-laden macrophages and is induced in monocyte-derived macrophages during cholesterol influx mediated by acetylated low-density lipoprotein.
In contrast, lipid efflux in cholesterol-laden macrophages, mediated by the cholesterol acceptor HDL(3), suppresses the expression of ABCG1.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Not recommended for Immunoprecipitation..
Other applications not tested.
Recommended Dilution:Western Blot: Mouse peritoneal macrophages treated with 22-R-hydroxycholesterol and T0901317 (stimulating ABCG1 expression).
Recognizes a band at ~75.6 kDOptimal dilution determined by the researcher.
Storage and Stability:Store product at 4°C, do not freeze.
Stable for 6 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human ABCG1. Species Crossreaactivity: Mouse. There is 100%/100% identity/homology of the immunogen used for this production and the human protein sequence. There is 84%/89% for mouse and 78%/88% for rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to an internal region of human ABCG1 (between residues 300-400).
