Proteinase 3 (PR3), a constituent of azurophil granules of neutrophils (polymorphonuclear cells, PMNs), is the target antigen for most anti-neutrophil cytoplasmic antibodies (c-ANCA) in Wegener’s Granulomatosis (WG).
Applications:Suitable for use in Western Blot, ELISA, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000 -1:2000Western Blot: 1:200-1:500Immunoprecipitation: 1:100 -1:200Immunohistochemistry: 1:50 -1:100Immunocytochemistry: 1:50- 1:100Optimal dilutions to be determined by the researcher
Note:In many cases, antigenicity of elastic fiber components can be enhanced by treating with 6M guanidine HCl, 50mM dithiothreitol, 20mM Tris pH 8.0 for 15 minutes.
After washing with 20mM Tris pH 8.0, the slides are treated with 100mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols..
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：250ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Whole serum
Form：Supplied as a lyophilized powder. Reconstitute with 250ul of sterile ddH2O. For long term storage, a preservative may be added.
Specificity：Recognizes human leucocyte proteinase 3.
