84-1109-72　Elastase, Pancreatic 500ul　E2236-21A

特徴

• Elastin is a protein in connective tissue that is elastic and allows many tissues in the body to resume their shape after stretching or contracting.
• Elastin helps skin to return to its original position when it is poked or pinched.
• Elastin is also an important load-bearing tissue in the bodies of mammals and used in places where mechanical energy is required to be stored.
• Elastin is particularly abundant in large elastic blood vessels such as the aorta.
• It is also very important in the lungs, elastic ligaments, the skin, the bladder, and elastic cartilage.
• Elastin is composed largely of glycine, proline, and other hydrophobic residues and contains multiple lysine-derived crosslinks, such as desmosines, which link the individual polypeptide chains into a rubberlike network.
• The hydrophobic regions of the chains, between the crosslinks, are highly mobile.
• The hydrophobic and crosslinking domains are coded by separate, small (27 to 114 bp) exons that are separated by large introns.
• The initial translation product is a 72kD polypeptide, designated tropoelastin.
• Elastin is made by linking many soluble tropoelastin protein molecules, in a reaction catalyzed by lysyl oxidase, to make a massive insoluble, durable cross-linked array.
• Applications:Suitable for Western Blot, ELISA, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:1000 -1:2000Western Blot: 1:200 -1:500Immunoprecipitation: 1:100 -1:200Immunohistochemistry/Immunocytochemistry: 1:50 -1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.