TARDBP was identified as the disease accumulating protein in patients with frontotemporal lobar degeneration (FTLD) with ubiquitin inclusions (FTLD-U) and in amyotrophic lateral sclerosis (ALS).
TARDBP functions as a DNA-binding protein and specifically binds to the TAR DNA sequence motifs of HIV.
Via this association with TAR motifs, TARDBP acts as a transcriptional repressor and inhibits HIV-1 transcription.
In addition, this protein regulates alternate splicing of the cystic fibrosis transmembrane receptor (CFTR) gene.
Applications:Suitable for use in Peptide ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Peptide ELISA: 1:16,000 Western Blot: 0.3-1ug/ml Detects ~45kD band in human Jurkat and NIH3T3 cell lysates.
A slightly larger protein is detected in some regins of the brain.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human TARDBP. Species crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to HISNAEPKHNSNRQ from the internal region of human TARDBP (NP_031401). Species Sequence Homology: canine, bovine, rat, porcine
