84-1108-79 FNIP1 (Folliculin interacting protein 1, DKFZp686E18167, DKFZp781P0215, KIAA1961, MGC667) 100ug F6002-66

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特徴

  • The FNIP1 gene consists of a 6655 nt mRNA (using NM 133372 derived from AC005593.1, DQ145719.1, AC008695.9 and AL832008.1, the coding sequence extends from nt143 to nt3643) and contains 18 coding exons.
  • The initiation codon is located within exon 1.
  • Northern blot analysis revealed an about 7 kb FNIP1 mRNA transcript that was expressed in most major adult tissues, with strongest expression in heart, liver and placenta, and expression in kidney and lung, tissues involved in the Birt-Hogg-Dube' syndrome phenotype (see below).
  • Several alternate transcripts lacking one or more exons have been reported.
  • Transcript 1 is the full-length isoform.
  • Transcript 2 lacks exon 7 (NM 001008738).
  • The FNIP1 protein contains 1166 amino acids and has an estimated molecular weight of 130kD.
  • Epitope-tagged FNIP1 expressed in HeLa cells localized exclusively in the cytoplasm.
  • Coimmunoprecipitation studies to elucidate the function of folliculin (FLCN) (encoded by the tumor suppressor gene, BHD/FLCN, which is mutated in the Birt-Hogg-Dube' syndrome) identified a novel folliculin-interacting protein, FNIP1, which interacts through the C-terminus of FLCN.
  • FNIP1 overexpression enhanced phosphorylation of FLCN and phospho-FLCN preferentially bound to FNIP1.
  • FNIP1 is a novel protein with no domains to suggest function.
  • By coimmunoprecipitation studies FNIP1 was also found to interact with the heterotrimer, 5'AMP-activated protein kinase (AMPK), a key molecule for energy sensing and a negative regulator of mTOR (mammalian target of rapamycin).
  • AMPK, which bound to FNIP1, was preferentially in its phosphorylated (active) form and FNIP1 could act as a substrate for AMPK phosphorylation both in vitro and in vivo.
  • Inhibition of AMPK kinase activity resulted in reduced FNIP1 protein expression in HEK293 cells suggesting that phosphorylation of FNIP1 by AMPK may enhance protein stability.
  • These data suggest that FNIP1 and its interacting partner, FLCN, may be involved in energy and nutrient-sensing through the AMPK and mTOR signaling pathways.
  • FNIP1 was also shown to interact with HSP90 by coimmunoprecipitation in HEK293 cells.
  • A comparison of FNIP1 proteins across species identified 5 blocks of conserved sequence with at least 35% similarity.
  • FNIP1 homologs have been identified in Mus musculus, Gallus gallus, Xenopus tropicalis, Danio rerio, Drosophila melanogaster and Caenorhabditis elegans.
  • FNIP1 interacts with FLCN, encoded by a novel tumor suppressor gene, BHD/FLCN, which is mutated in the germline of patients with BHD syndrome.
  • Genodermatosis characterized by the triad of benign tumors of the hair follicle, spontaneous pneumothorax and kidney tumors.
  • Applications:Suitable for use in ELISA.
  • Other applications not tested.
  • Recommended Dilution:Peptide ELISA: 1:32,000.
  • Western Blot: Preliminary experiments in Human Heart, Liver, Ovary and Spleen lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).
  • Optimal dilutions to be determined by the researcher.
  • Storage and Stability:May be stored at 4°C for short-term only.
  • For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
  • Aliquots are stable for at least 12 months at -20°C.
  • For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
  • Further dilutions can be made in assay buffer.
  • Peptide Blocking:Corresponding peptide is available for Peptide Blocking studies.
  • See F6002-66-P.
  • Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet.
  • When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml.
  • To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed).
  • Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature.
  • It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min.
  • Then incubate and develop the two blots in parallel.

仕様

  • Size:100ug
  • Host:goat
  • Source Antibody:human
  • Grade:Affinity Purified
  • Purity:Purified by affinity chromatography.
  • Form:Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
  • Specificity:Recognizes human FNIP1.
  • Isotype:IgG
  • Calc Applications Abbrev:E
  • Calc Crossreactivity:Hu
  • Immunogen:A synthetic peptide from the C-terminus of aa-ENISQQEREDIQN
  • Shelf Life:1year
  • EU Commodity Code:30021010
  • この商品は法規制を確認しておりません。(法規制によって販売できない場合もございます)
  • 製品の仕様は予告なく変更になる場合がございます。最新仕様はメーカーホームページをご確認ください。
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アズワン品番 84-1108-79
型番 F6002-66
入り数 1個
標準価格 117,000円(税抜)
WEB価格
出荷予定日
アズワン在庫 [?]
サプライヤ在庫 [?]
数量

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