Applications:Suitable for use in Immunohistochemistry, ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Peptide ELISA: 1:32,000.
Western Blot: 0.5-1.5ug/ml.
A band at ~170kD band observed in lysates of cell line Jurkat
Immunohistochemistry: 3-6ug/ml.
In paraffin embedded Human Kidney shows nuclear staining in PCT
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human DNMT1.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-RFESPPKTQPTEDN from an internal region of human DNMT1. Species sequence homology: mouse, rat, canine, bovine and porcine.
