Common Alzheimer's disease risk variant within the CLU gene affects white matter microstructure in young adults.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Peptide ELISA: 1:8000.
Western Blot: 0.3-1ug/ml.
A band is detected at ~37kD in Mouse Brain and Testis lysates and in Rat Brain and Ovary lysates (calculated MW of 51.6kDa according to Mouse NP_038520.1).
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can
Peptide Blocking:Corresponding peptide is available for Peptide Blocking studies.
See C5870-01C-P.
Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet.
When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml.
To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed).
Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature.
It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min.
Then incubate and develop the two blots in parallel.
仕様
Size：100ug
Host：goat
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes rat Clusterin. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo Rt
Immunogen：Synthetic peptide corresponding to the C-terminus of EKALQEYRRKSRAE. Species sequence homology: mouse.
