C1D is known as nuclear nucleic acid-binding protein C1D.
C1D is a ubiquitously expressed nuclear matrix DNA-binding, and apoptosis-inducing protein, which acts as an activator of the DNA-dependent protein kinase (DNA-PK), and is implicated as a regulator of TRAX/Translin complex formation.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:Peptide ELISA: 1:28,000.
Western Blot: 1-3ug/ml.
Approx.
14kD band observed in Human Skeletal Muscle lysates (calculated MW of 16.0kD according to NP_775269.1 and NP_775269.1).
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Peptide Blocking:Corresponding peptide is available for Peptide Blocking studies.
See C2777-84-P.
Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet.
When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml.
To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed).
Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature.
It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min.
Then incubate and develop the two blots in parallel.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human C1D.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：A synthetic peptide from the C-terminus of aa-SKNASKVANKGKSKS.
