SHPRH is a member of the SWI/SNF family of ATPaes/helicases, it posesses a C3HC4 RING motif characteristic of ubiquitin ligase protein and it has a fundamental role in DNA repair mechanism.
Upon genotoxic stress, SHPRH associates with PCNA, RAD18, and the ubiquitin-conjugating enzyme UBC(E2) and promotes methyl methanesulfonate (MMS)-induced PCNA polyubiquitation suppressing genomic instability.
SHPRH gene is mutated in a number of cancer cell lines, including those from melanomas and ovarian cancer.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:500Western Blot: 1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2.
Specificity：Recognizes human SHPRH
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide (DKTKKQAVGSPRKIQKETRK) corresponding to aa 521-540 of human SHPRH
