Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue.
Human PDGF-BB is a 24.3kD B chain homodimer protein containing 109 amino acid residues.
PDGF-BB (Platelet derived growth factor B chain), a disulphide-linked beta-chain homodimer and member of the PDGF family, released from platelet alpha-granules following trauma/platelet activation.
Naturally occurring active forms of PDGF exist as dimers of two alpha chains (AA), two beta chains (BB), or a combination of both chains (AB), all of which signal through the high-affinity PDGF-Ralpha receptor, and the latter two of which can also signal through the PDGF-Rbeta receptor.
PDGFs are potent mitogens for a variety of cells including those of the connective tissue, bone and cartilage, blood and smooth muscle cells, and are involved in several biological processes including chemotaxis, hyperplasia and embryonic neurogenesis.
The expression of PDGF-BB is associated with autocrine growth stimulation and the formation of various tumor types, with several studies focusing on dermatofibrosarcoma protuberans (DP), an infiltrating skin tumor arising from a translocation occurring between chromosome 22 (the location of the PDGFB gene) and chromosome 17 (the location of the COL1A1 gene), which results in the generation of the COL1A1-PDGFB chimeric protein.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-HCl, pH 7.5.
