N-Myc is a 49.5kD nuclear phosphoprotein that contains a basic helix-loop-helix leucine zipper domain involved in DNA binding and protein dimerization.
It regulates gene expression by dimerizing with itself or other helix-loop-helix proteins and this complex binds to DNA E-box sequences of regulated genes.
N-Myc is expressed in high levels during embryonic development and in the adult during B-cell differentiation.
It is overexpressed in a variety of tumors, aminly neuroblastomas and is associated with rapid progression and poor prognosis.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:500Western Blot: 1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2.
Specificity：Recognizes human N-Myc
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide (SPYVESEDAPPQKK) corresponding to aa337-349 of human N-Myc
