Cullins (1, 2, 3, 4a, 5 and 7) are a family of proteins that assemble a large number of ubiquitin ligases to catalyse polyubiquitiation, as well as binding to various specific factors to recruit substrates.
Cullin 4 (CUL 4) serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits of SCF.
It plays a role in the polyubiquitination of CDT1 in response to radiation-induced DNA damage and may be important for subsequent DNA repair.
Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:500Western Blot: 1:1000Immunocytochemistry: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2.
Specificity：Recognizes human CUL 4a
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide (MADEAPRKGSFSALVGRTNG) corresponding to aa1-20 of human Cullin 4a
