Cullins (1, 2, 3, 4a, 5 and 7) are a family of proteins that assemble a large number of ubiquitin ligases to catalyse polyubiquitiation, as well as binding to various specific factors to recruit substrates.
Cullin 2 (CUL 2) is a component of E3 ubiquitin ligase complexes which controls the proteolysis of G1 cell cycle regulatory proteins.
It forms a complex with an inactive transcriptional elongation complex formed by three subunits elongain C, elongain B and VHL.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:100Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2.
Specificity：Recognizes human CUL 2
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide (RSQASADEYSYVA) corresponding to aa733-745 of human Cullin 2
