84-1107-39　［取扱停止］YAP (65kD Yes-associated Protein, YAP 65, YAP65, YAP-65, Yes Associated Protein 1 65kD, Yes-associated Protein 1, YAp 1, YAP1, YAP2) 100ul　Y1200-01D

特徴

• YAP (Yes-Associated Protein) is a founding member of the WW domain family of proteins, transcriptional co-activator and oncogene.
• Its gene is transcriptionally activated in squamous cell carcinoma cells.
• As one of several genes of 1q22 amplicon, YAP was recently implicated in liver and breast cancer.
• Overexpression of YAP gene in MCF10A mammary cell line induces oncogenic transformation.
• Applications:Suitable for use in ChIP, Immunohistochemistry, Immunoprecipitation, Western Blot and Immunofluorescence/Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Immunofluorescence (IC): 1:500Immunohistochemistry (Frozen/Paraffin): 1:50-1:200Western Blot: 1:1000.
• Detects 65kD band using transfected HEK 293 lysate as sample
• Immunoprecipitation: 2-10ugOptimal dilutions to be determined by the researcher.
• Positive Control:Brain and kidney lysates and HEK 293 cell lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.