The von Hippel-Lindau (VHL) protein is a tumor suppressor that is highly conserved.
VHL binds to transcription factor Elongin B and C subunits, and appears to prevent Elongin A from associating with the B and C subunits, thereby inhibiting the transcriptional activity of Elongin.
The ability of VHL to block Elongin function may play a role in normal cell growth regulation.
Mutated VHL sequences lose their ability to bind to Elongin subunits B and C, suggesting that the tumor suppression function of VHL may be linked to its ability to bind to Elongin.
Likewise, the introduction of wild-type VHL suppresses growth in tumor cell lines lacking normal expression of VHL genes.
The product of the VHL gene has multiple reported functions, the best characterized of which is its role as the recognition component of an ubiquitin E3 ligase complex responsible for mediating oxygen-dependent destruction of hypoxia-inducible factor-alpha (HIF-alpha) subunits.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 3 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Specificity：This antibody is specific for VHL protein. Species Crossreactivity: This antibody reacts with the human VHL protein. The sequence used for immunization is divergent from mouse protein and, therefore, it is unlikely that this antibody will react with mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：A synthetic peptide derived from the human VHL protein.
