TLR4 requires the binding of MD-2 to its extracellular region for best response to bacterial LPS.
MD-2 also enhances TLR4-dependent activation of NF-kappa-beta and cooperates with TLR2 in the response to cell wall components from bacteria.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/ml, detects a band of ~26kD in mouse spleen cell lysateImmunohistochemistry (Paraffin): 2ug/mlOptimal dilutions to be determined by the researcher.
Immunohistochemistry Positive Control Tissue:Rat spleen
Recommended Secondary Antibodies:Goat Anti Rabbit IgG (H&L) HRPSheep Anti Rabbit IgG BiotinGoat Anti Rabbit IgG (Fc) FITC, HRPGoat Anti Rabbit IgG (H&L) HRPSheep Anti Rabbit IgG FITCSheep Anti Rabbit IgG RPESheep Anti Rabbit IgG DyLight™488, DyLight™549, DyLight™649Sheep Anti Rabbit IgG HRP
Immunohistochemistry Positive Control Tissue:Rat spleen
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified by Ion Exchange chromatography from whole serum.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human MD-2. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids near the middle of human MD-2.
