CD82 has been identified as a cancer metastasis suppressor protein, while ubiquitously expressed at moderate to high levels in many normal tissues, its expression is down regulated during tumor progression.
Loss of CD82 and p53 is correlated with poor prognosis for patients with prostate and other forms of cancer.
Research has demonstrated that CD82 interacts with CD234 (DARC).
It has been hypothesized that tumor cells dislodged from the primary tumor and expressing CD82 interact with CD234 expressed on vascular cells, transmitting a senescent signal to the tumor cells.
Tumor cells that have lost CD82 expression can proliferate and potentially give rise to metastases.
It has been demonstrated that at least two isoforms of CD82 exist.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlImmunohistochemistry (Paraffin): 2.5ug/mlOptimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesSheep Anti Rabbit IgG HRP
Immunohistochemistry Positive Control Tissue:Human colon
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes the human CD82 antigen in human colon cell lysates at ~ 33kD.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 15 aa sequence from near the carboxy terminus of Human CD82.
