84-1106-31　SREBP-1 (Sterol Regulatory Element Binding Protein-1, SREBP1, ADD1, D630008H06, Sterol Regulatory Element Binding Transcription Factor 1, SREBF1) 100ul　S6505-15A

特徴

• SREBP-1 and-2 are transcription factors which participate in the control of cholesterol homeostasis.
• SREBPs proteins are attached to the endoplasmic reticulum and nuclear envelope.
• They are proteolytically cleaved and activated in response to conditions of low cellular sterol.
• Upon activation of SREBP-1 or -2, an ~480-500aa, N-terminal cleavage fragment of these proteins enters the nucleus, activating transcription of enzymes and other proteins required for cholesterol synthesis.
• SCA (SREBP-cleavage activity) and caspase-3 proteases cleave SREBPs.
• SREBP proteins containing point mutations at caspase-3 cleavage sites (Asp460 in SREBP-1 and Asp468 in SREBP-2) do not become cleaved following induction of apoptosis.
• This suggests that SREBPs may play some role in apoptotic processes.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence/Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:500.
• Detects a band at ~65kD
• Immunohistochemistry (Frozen and paraffin): 1:200-1:500Immunofluorescence (IC): 1:200Optimal dilutions to be determined by the researcher.
• Positive Control:Mouse liver whole tissue lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.