Mammalian amino acid transport systems consist of a large variety of transporters.
The amino acid transporter, cystine/glutamic acid transporter xCT, for system xc has been proposed to be responsible for the cystine transport through the plasma membrane.
System xc mediates an amino acid exchange and prefers cystine and glutamate as its substrates.
The transporter designated as xCT requires 4F2 heavy chain (4F2hc:CD98) for its functional expression, and belongs to the family of amino acid transporters that associates with the type II membrane glycoproteins such as 4F2hc.
In brain, it has been proposed that system xc is up-regulated in glial cells upon oxidative stress and plays an essential roles to protect neurons against oxidative stress.
Recent studies have implicated xCT as a gateway that Kaposi's sarcoma Herpes virus (KSHV) uses to enter cells.
Applications:Suitable for use in Western Blot and Immunohistochemistry (paraffin-embedded sections).
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Positive Control: Stomach tissue lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Tris-glycine with 150mM NaCl
Specificity：This antibody is specific for XCT. Species Crossreactivity: This antibody reacts with the human and mouse proteins. Other species have not been tested.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：A synthetic peptide made to a region within the N-terminus of the human XCT protein sequence (between residues 1-50).
