B5R, a 561 amino acid membrane protein containing ankyrin repeats that mediate protein-protein interactions and is related to the regulators of complement activation (RCA) superfamily.
It consists of four short consensus repeats (SCRs) and transmembrane anchor region or the CT (cytoplasmic tail).
Its transmembrane domain is the major determinant for targeting the B5R protein to the outer membrane of EEV (extracellular enveloped virus) and for supporting EEV formation, thus playing an important role in the process of virus envelopment and release.
It may also be involved in viral evasion from host immune responses.
B5R is essential for efficient wrapping of IMV, actin tail formation, normal plaque size, virus virulence, incorporation of the protein into EEV and for EEV formation.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-1.0ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes Smallpox B5R. Species Crossreactivity: human and bovine. Species sequence homology: camel (93%), monkey and rabbit (80%).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Hu
Immunogen：Synthetic peptide corresponding to aa33-47, IQEYNLIRTYKKVDK, of B5R protein.
