SMADs are members of the MAD-related family of molecules.
MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily (1).
SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD: SMAD4) and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7).
SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them.
SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.
Human SMAD1 is a 465aa protein.
Human SMAD2 is a 467aa protein.
Human SMAD3 is a 425aa protein.
Human SMAD4 is a 552aa protein.
Human SMAD5 is a 465aa protein.
Human SMAD6 is a 496aa protein.
Human SMAD7 is a 426aa protein.
Mouse SMAD8 is a 430aa protein.
Human SMAD9 is a 430aa protein.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：25ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human Smad1, Smad5, Smad8, Smad9. May crossreact with SMAD2 or SMAD3. The actual SMADs detected may vary according to cell or tissue-type specific expression. Species Crossreactivity: Mouse, rat and chicken.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ch Hu Mo Rt
Immunogen：Two synthetic peptides corresponding to aa18-32 and aa315-330 ((LGWKQGDEEEKWAEK); (SNVNRNSTIENTRRHI) from human SMAD1 (GenBank Accession #AAP36050.1). Sequence Homology (100%): SMAD5, SMAD8, SMAD9.
Shelf Life：1year
Swiss Prot Number：Q15797
EU Commodity Code：30021010
商品名：Smad1, Smad5, Smad8, Smad9 (SMAD-1, SMAD-5, SMAD-8, SMAD8A, SMAD8B, SMAD-9, BSP1, BSP-1, CMT2D, DPC4, GARS, HMN5, hSMAD1, hSMAD4, JV41, JV51, MAD Homolog 5, Mad-related Protein 1, MADR1, Madh6, Mothers Against Decapentaplegic Homolog 1, Mothers Against DPP Homolog 1, MADH1, Mothers Against Decapentaplegic Homolog 5, Mothers Against DPP Homolog 5, MADH5, Mothers Against Decapentaplegic Homolog 9, Mothers Against DPP Homolog 9, MADH9, Transforming Growth Factor beta Signaling Protein 1, TGF beta Signaling Protein 1) 25ug
