One family of inhibitory axon guidance molecules is the semaphorins.
The semaphorins include secreted, transmembrane, and GPI anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets.
SEMA 3B inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Positive Control: Mouse E16 cerebellum lysate
Cellular Localization: Secreted.
Accumulates in the endoplasmic reticulum.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris-glycine, 150mM sodium chloride, pH 7.2.
Specificity：This antibody is specific for SEMA 3B. Species Crossreactivity: This antibody reacts with mouse SEMA 3B protein. The immunogen used for this antibody production has 100% homology with human, 92% with mouse, 90% with Zebrafish, and 90% homology with human, mouse, and rat SEMA 3A protein. There is no significant homology with rat protein.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Ze
Immunogen：A synthetic peptide made to an internal portion of the human SEMA 3B protein sequence (between residues 100-200).
