84-1105-95　Scavenger Receptor BI/BII (Scavenger Receptor-B1/B2, SR-BI/BII, SR-B1/B2, Scavenger Receptor Class B Type I, SCARB1, SRBI, Scavenger Receptor Class B Member 2, SRBII, SCARB2, 85kD Lysosomal Membrane Sialoglycoprotein, CD36 and LIMPII Analogous 1, CLA1, CD36L1, CD36L2, HLGP85, LGP85, Lysosome Membrane Protein II, LIMP II) 100ul　S0099-02C

特徴

• High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and their plasma concentrations are inversely correlated with risk for atherosclerosis.
• SR-BI and SR-BII (previously known as SR-BI.2) are the alternatively spliced products of a single gene.
• SR-BII and SR-BI are identical except for the encoded c-terminal cytoplasmic domain.
• Both SR-BI and SR-BII bind HDL and mediates selective uptake of HDL cholesteryl ester, but with SR-BII having an approximately 4-fold lower efficiency than SR-BI.
• SR-BI and SR-BII are expressed primarily in liver and non-placental steroidgenic tissues.
• Although the role of these scavenger receptors is not completely clear, SR-BII mRNA results from the alternative splicing of SR-BI precursor transcripts with both isoforms mediating selective transfer of lipid between HDL and cells.
• Therefore, the relative expression and functional activities of these two isoforms create a potential means of regulating selective lipid transfer between HDL and cells.
• Applications:Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, Immunoprecipitation and Immunoflourescence/Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Flow Cytometry: 1:400Western Blot: 1:1000 detects a band of ~82kD in tissues that express SR-BI and/or SR-BII such as liver, ovary, adrenal glands and to as lesser extent testes, heart and mammary glands.
• Immunohistochemistry: 1:10-1:2000 Immunofluorescence/Immunocytochemistry: 1:50-1:1000 Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.