The genus Salmonella is a member of the family Enterobacteriaceae.
The genus is composed of Gram negative bacilli that are facultative and flagellated (motile).
Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae).
Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria.
This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A.
Endotoxins evoke fever and can activate complement, kinin and clotting factors.
Applications:Suitable for use in ELISA, Immunocytochemistry/Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1;100-1:2000Immunofluorescence (IC): 1:10-1:500Immunohistochemistry (Frozen/Paraffin): 1:10-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes Salmonella. Polyvalent for Salmonella O and H antigens. Species Crossreactivity: mouse. Does react with related Enterobacteriaceae.
Isotype：IgG
Calc Applications Abbrev：E IC IF IHC
Calc Crossreactivity：Mo
Immunogen：Mixture of S. enteriditis, S. typhimurium and S. heidelburg. Cellular Localization: Whole cell
