S100A12 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs.
S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation.
S100A12 is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities.
S100A12 is also a ligand for RAGE.
Interaction with RAGE on mononuclear phagocytes, lymphocytes and endothelium triggers cellular activation.
Nuclear factor kappa B, a key transcription factor for inflammatory events, is activated by S100A12/RAGE.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot 1:1000, Western blot, where a band is seen ~14 kD
Other applications have not been tested.
Positive Controls:HeLa whole cell extract
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.09% sodium azide.
Specificity：Species Crossreactivity: human. Not reactive with mouse or rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Full length human S100A12 protein [Swiss-Prot# P80511] expressed in E. coli. Cellular Localization: nuclear and cytoplasm.
