TPMT (thiopurine S-methyltransferase) is an enzyme that catalyzes the S-methylation of aromatic and heterocyclic sulfhydryl compounds, including the immunosuppressant azathioprine (AZA) and the antineoplastic agents 6-mercaptopurine (6MP) and 6-thioguanine (6TG).
The enzyme is also involved in the normal metabolic inactivation of thiopurine drugs.
Patients with intermediate or no TPMT activity are at risk of toxicity after receiving standard doses of thiopurine drugs.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.05-0.2ug/ml Western Blot: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：40ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.4, 0.02% sodium azide.
Specificity：Recognizes human TPMT protein.
Isotype：IgG
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide (KLH). within residues 200-250 of human TPMT protein (Swiss Prot: P51580).
