Tau is a microtubule-associated phosphoprotein (MAP), localized in neuronal axons.
It promotes tubulin polymerization and stabilizes microtubules.
Tau proteins constitute a family of six isoforms, which range from 352 to 441 amino acids.
The tau variants differ from each other by the presence of either three or four repeat-regions in the carboxy-terminal part of the molecule and the absence or presence of one or two inserts in the amino-terminal part
Tau is hyperphosphorylated by ERK, GSK-3, TPKII, and CDK5.
At least thirty phosphorylation sites have been described, including Thr39, Ser46, Thr50, Thr69, Thr153, Thr175, Thr181, Ser198, Ser199, Ser202, Thr205, Ser208, Ser210, Thr212, Ser214, Thr217, Thr231, Ser235, Ser237, Ser241, Ser262, Ser285, Ser305, Ser324, Ser352, Ser356, Ser396, Ser400, Thr403, Ser404, Ser409, Ser412, Ser413, Ser416, and Ser422.
Specifically, TPKII phosphorylates serines 202 and 404.
GSK-3β transfection phosphorylates serines 199, 202, 235, 396, 404 and 413, and threonines 205 and 231.
These sites are among the major abnormal phosphorylation sites of Tau.
Phosphorylation on these sites reduces the ability of a given Tau species to promote microtubule self-assembly.
Hyperphosphorylated Tau is the major protein of the paired helical filaments (PHFs), which make up the pathological neurofibrillary tangles of Alzheimer’s disease (AD).
These PHFs are also found in the lesions of other central nervous system disorders.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 0.05-0.2ug/mlWestern Blot: 0.1-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：40ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 30% glycerol
Specificity：Recognizes endogenous levels of human Tau only when phosphorylated at threonine 217. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phospho-peptide corresponding to residues surrounding Thr217 of human Tau, conjugated to KLH
