Applications:Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:1000Immunohistochemistry (Paraffin): 1:50-1:100Immunofluorescence: 1:100-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 0.02% sodium azide, 50% glycerol
Specificity：Recognizes endogenous levels of human Shc1 only when phosphorylated at tyrosine 427.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthesized phosphopeptide derived from human Shc1 around the phosphorylation site of tyrosine 427 (P-S-YP-V-N).
