HT receptors interact with the neurotransmitter and peripheral signal mediator serotonin.
In particular, 5-hydroxytryptamine (5HT) receptors mediate the role of serotonin as an endogenous ligand and in pharmaceutical and hallucinogenic drugs.
In addition, 5HT receptors affect the release and activities of other neurotransmitters, such as glutamate, dopamine, and GABA.
5HT3A receptor belongs to the serotonin receptor family and is one member of G protein coupled receptor (GPCR) family.
The receptor is a ligand-gated ion channel that mediates a fast excitatory response.
This receptor is also a member of the ligand-gated ion channel superfamily and is related to the nicotinic, GABAA, and NMDA receptors.
Applications:Suitable for use in ELISA, Western Blot and Flow Cytometry.
Other applications not tested.
Recommended Dilutions:ELISA: 0.05-0.2ug/mlWestern Blot: 0.5-1ug/mlFlow Cytometry: 2-5ug for 1x10e6 cellsOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：40ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitute with 80ul sterile ddH2O.
Specificity：Recognizes endogenous levels of human 5HT3A protein. Species crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E FC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to the first extracellular domain of human 5-HT3A, conjugated to KLH (Swiss Prot: P46098).
