84-1105-23　［取扱停止］Protein Kinase C, mu (PKCu) 40ug　P9103-59A

特徴

• Protein kinase C ('PKC', EC 2.7.11.13) is a cyclic, nucleotide-independent enzyme that phosphorylates serine and threonine residues.
• The PKC family is divided into three groups depending on each the enzymes' cofactor requirements: conventional (c)PKC isoforms (α, βI, βII, and γ) require diacylglycerol (DAG), Ca2+, and phospholipids for activation; novel (n)PKC isoforms (δ, ε, η (also known as PKC-L), θ, and µ the mouse homolog of human PKCµ (also known as PKD)) require DAG but not Ca2+ for activation; and atypical (a)PKC isoforms, (ζ, ι, and λ, the mouse homolog of human PKC ι) require neither Ca2+ nor DAG.
• A new PKC member has recently been discovered and is referred to as PKCν
• PKC's phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways.
• These kinases also serve as major receptors for phorbol esters, a class of tumor promoters.
• Every member of the PKC family has a specific expression profile and is believed to play a distinct role in cells
• PKCμ, also known as nPKC-D1 and nPKC-mu, is a member of PKC family of serine/threonine kinases.
• PKCμ has two characteristic cysteine-rich repeats, a conserved ATP-binding consensus sequence in the kinase domain, and the invariate aspartate essential for kinase activity.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.05-0.2ug/mlWestern Blot: 0.5-2ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.