84-1105-21　［取扱停止］Protein Kinase C, zeta (PKCz) 40ug　P9103-40Y

特徴

• Protein kinase C ('PKC', EC 2.7.11.13) is a cyclic nucleotide-independent enzyme that phosphorylates serine and threonine residues in many target proteins.
• The PKC family has been divided into three groups differing in the enzymes' cofactor requirements: conventional (c)PKC isoforms (comprising α, βI (also known as β1), βII (also known as β2) and γ)), that require diacylglycerol (DAG), Ca2+, and phospholipid for activation; novel (n)PKC isoforms (comprising δ, ε, η (also known as PKC-L), θ and µ (the mouse homolog of human PKCµ, known as PKD)) that require DAG but not Ca2+; and atypical (a)PKC isoforms, namely ζ, ι and λ (the mouse homolog of human PKC ι) that require neither Ca2+ nor DAG.
• A new PKC member has recently been discovered and is referred to as PKCν
• PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways.
• PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters.
• Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.
• PKCζ (PKC zeta) exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine.
• Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester.
• Unlike the classical PKC isoenzymes, it has only a single zinc finger module.
• These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.05-0.2ug/mlWestern Blot: 0.5-2ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:For long-term storage, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.