84-1105-20　［取扱停止］Protein Kinase C, gamma (PKCg) 40ug　P9103-25V1

特徴

• Protein kinase C ('PKC', EC 2.7.11.13) is a cyclic nucleotide-independent enzyme that phosphorylates serine and threonine residues in many target proteins.
• It is involved in diverse cellular signaling pathways.
• Specifically, PKC family members serve as major receptors for phorbol esters, a class of tumor promoters.
• Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells
• PKC family is divided into three groups based on different enzymes' cofactor requirements.
• They are conventional (c) PKC isoforms, novel (n) PKC isoforms, and atypical (a)PKC isoforms respectively.
• Conventional (c) PKC isoforms consisting α, βI, βII and γ require diacylglycerol (DAG), Ca2+, and phospholipid for activation.
• Novel (n)PKC isoforms consisting δ, ε, η (also known as PKC-L), θ and µ (the mouse homolog of human PKCµ, known as PKD) require DAG but not Ca2+ for activation.
• Atypical (a)PKC isoforms, consisting ζ, ι and λ (the mouse homolog of human PKC ι) require neither Ca2+ nor DAG.
• Other than those listed above, a new PKC member has recently been discovered and is referred to as PKCν.
• PKCγ (PKC gamma) plays a key role in neuronal signal transduction and regulating intercellular communication.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5-2ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.