Protein kinase C ('PKC', EC 2.7.11.13) is a cyclic nucleotide-independent enzyme that phosphorylates serine and threonine residues in many target proteins.
It is involved in diverse cellular signaling pathways.
Specifically, PKC family members serve as major receptors for phorbol esters, a class of tumor promoters.
Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.PKC family is divided into three groups based on different enzymes' cofactor requirements.
They are conventional (c) PKC isoforms, novel (n) PKC isoforms, and atypical (a)PKC isoforms respectively.
Conventional (c) PKC isoforms consisting a, bI, bII and g require diacylglycerol (DAG), Ca2+, and phospholipid for activation.
Novel (n)PKC isoforms consisting d, e, eta (also known as PKC-L), th and µ(the mouse homolog of human PKCµ, known as PKD) require DAG but not Ca2+ for activation.
Atypical (a)PKC isoforms, consisting sd, i and l (the mouse homolog of human PKC ι) require neither Ca2+ nor DAG.
Other than those listed above, a new PKC member has recently been discovered and is referred to as PKCν.
PKCν (PKC nu), also known as Protein kinase D3 (PKD3), is widely expressed in human and murine tissue.
It is stimulated by both phorbol ester and growth factors and is phosphorylated upon stimulation at Ser731 and Ser735, located within the activation loop.
PKD3 continuously shuttles between the nucleus and the cytoplasm, which requires a functional CRM1 nuclear export pathway.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.05-0.2ug/mlWestern Blot: 0.5-2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute by adding sterile 40-50% glycerol, ddH2O.
Reconstituted product is stable for 12 months at -20ºC.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：40ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide.
Specificity：Recognizes endogenous human PKCg protein. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide derived from human PKCg. (KLH).
