Protein kinase C ('PKC', EC 2.7.11.13) is a cyclic nucleotide-independent enzyme that phosphorylates serine and threonine residues in many target proteins.
The PKC family has been divided into three groups by the enzymes' cofactor requirements: conventional (c)PKC isoforms (comprising a, bI (also known as b1), bII (also known as b2) and g), that require diacylglycerol (DAG), Ca2+, and phospholipid for activation; novel (n)PKC isoforms (comprising d, e, eta (also known as PKC-L), Phi and µ (the mouse homolog of human PKCµ, known as PKD)) that require DAG but not Ca2+; and atypical (a)PKC isoforms, namely zeta, iota, and lambda (the mouse homolog of human PKC ι) that require neither Ca2+ nor DAG.
A new PKC member has recently been discovered and is referred to as PKCν.
PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways.
PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters.
Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.
PKC activity is regulated through three distinct phosphorylation sites, the activation loop threonine (Thr497 in PKCa and Thr500 in PKCb2), the turn loop autophosphorylation threonine (Thr638 in PKCa and Thr641 in PKCb2) and the hydrophobic loop serine (Ser657 in PKCa and Ser660 in PKCb2).
Atypical PKC isoforms lack hydrophobic region phosphorylation, which correlates with the presence of glutamic acid rather than the serine or threonine residues found in more typical PKC isoforms
Applications:Suitable for use in ELISA and Western Blot Other applications not tested.
Recommended Dilution:ELISA: 0.05-0.2ug/mlWestern Blot: 0.5-2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：40ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.4, 0.02% sodium azide.
Specificity：Recognizes endogenous levels of total human and mouse PKCα and PKCβ2 protein.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic non-phosphopeptide (KLH). corresponding to residues surrounding Thr638 of human PKCα and Thr641 of human PKCβ2.
