84-1105-12　［取扱停止］Protein Kinase A II alpha, Regulatory Subunit (Protein Kinase A RII alpha Subunit, Protein Kinase cAMP-dependent Regulatory Type II alpha, PRKAR2A, PRKAR2, PKR2, KAP2, MGC3606) 40ug　P9102-91M3

特徴

• The cAMP-dependent protein kinase (PKA) is a critical kinase involved in numerous biological functions.
• The PKA holoenzyme is composed of two regulatory and two catalytic subunits designated as PKA R and PKA C respectively.
• PKA R subunits exist as two classes, RI and RII, each containing two isoforms, α and β.
• On the other hand, PKA C has three identified subunit isoforms, Cα, Cβ, and Cγ, have been identified.
• The PKA R subunits allow a range of modifying agents to influence PKA activity and localization.
• They are found in different levels with tissue specificity.
• Following the bindings between PKA R subunits and the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates many cellular functions such as metabolism, ion transport, and gene transcription.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5-2ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.