The cAMP-dependent protein kinase (PKA) is a critical kinase involved in numerous biological functions.
The PKA holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively.
PKA regulatory (PKA R) subunits exist as two classes, RI and RII, with each class containing two isoforms, α and β.
Three catalytic subunit isoforms, Cα, Cβ, and Cγ, have been identified.
The PKA R subunits allow a range of modifying agents to influence PKA activity and localization, and are found in different levels in a tissue-specific manner.
Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.05-0.2ug/mlWestern Blot: 0.5-2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：40ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 30% glycerol.
Specificity：Recognizes endogenous levels of human and mouse PKA regulatory subunit RIIβ (PKA RIIβ) protein.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide (KLH). derived from human PKA regulatory IIβ subunit (PKA RIIβ)
