It is involved in nucleosome wrapping and the compaction of DNA into chromatin, limiting DNA accessibility.
Histones thereby play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
DNA accessibility is regulated via a complex system of nucleosome remodelling and post-translational modification of histones, also called histone code.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.05-0.2ug/mlWestern Blot: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：40ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.4, 0.02% sodium azide.
Specificity：Recognizes endogenous levels of human histone 2B protein.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide (KLH). derived from the carboxyl terminal of human histone 2B (Swiss Prot: Q99879).
