It stabilizes existing proteins against aggregation and mediates the folding of newly translated polypeptides in both cytosol and organelles.
HSP70 participates in all of these processes through its ability to recognize nonnative conformations of other proteins.
It binds extended peptide segments with a net hydrophobic character exposed during translation and membrane translocation or following stress-induced damage.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.05-0.2ug/ml Western Blot: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute by adding sterile 40-50% glycerol, ddH2O.
Reconstituted product is stable for 12 months at -20ºC.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：40ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide.
Specificity：Recognizes endogenous levels of human HSP70 protein. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide within residues 350-400 of human HSP70 protein (Swiss Prot: P08107). (KLH).
