Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is one of the key enzymes involved in glycolysis.
Though expressed differently in different tissues , GAPDH is thought to be a constitutively expressed housekeeping protein.
For this reason, GAPDH is often measured as a control in experiments quantifying specific changes in expression of other targets.
Recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair.
GAPDH may also play a role in neurodegenerative pathologies such as Huntington's and Alzheimer's disease.
Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-1.0ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：40ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 30% glycerol. Labeled with Biotin.
Specificity：Recognizes GAPDH. Species Crossreactivity: human, mouse, rat, goat, rabbit, chicken, hamster, bovine, swine, fish, and E. coli cell or tissue lysate
