Adherens junctions (also called AJs and the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces.
AJs mediate adhesion between cells, communicate signals that neighboring cells are present, and anchor the actin cytoskeleton.
In serving these roles, AJs regulate normal cell growth and behavior.
At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia.
This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.05-0.2ug/mlWestern Blot: 0.5-2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：40ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.4, 0.02% sodium azide.
Specificity：Recognizes human b-catenin protein.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide within residues 30-80 of human b-catenin protein (KLH). (Swiss Prot: P35222).
