The alpha-, beta- and gamma-catenins are cytoplasmic proteins mediating the interaction of Ca2+ -dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network.
The N-terminus of alpha-catenin directly interacts with exon 3 of beta-catenin.
The C-terminus of alpha-catenin interacts with the actin filament network.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human alpha-catenin only when phosphorylated at serine 641. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic phosphopeptide derived from human alpha-catenin around the phosphorylation site of serine 641 (D-D-SP-D-F)
