Calnexin is calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum.
It may be involved in assisting protein assembly or in the retention within the ER of unassembled protein subunits.
It seems to play a major role in the ER quality control apparatus via the retention of incorrectly folded proteins.
Applications:Suitable for use in ELISA, Western Blot and Flow Cytometry.
Other applications not tested.
Recommended Dilutions:ELISA: 0.05-0.2ug/mlWestern Blot: 0.5-1ug/mlFlow Cytometry: 1-3ug to label 1x10e6 cellsOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：40ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitute with 80ul sterile ddH2O.
Specificity：Recognizes endogenous levels of human calnexin protein. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E FC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide from the carboxyl terminal of human calnexin protein, conjugated to KLH (Swiss Prot: P27824). Species Sequence Homology: rat, bovine, canine and Sumatran orangutan
