b-secretase, also called BACE (b-site of APP cleaving enzyme), is an aspartic-acid protease important in the pathogenesis of Alzheimer's disease, and in the formation of myelin sheaths in peripheral nerve cells.
BACE1, also known as memapsin-2, is the major b-secretase for generation of the 40 or 42 amino acid-long b-amyloid peptides that aggregate in the brain of Alzheimer's patients.
Extracellular cleavage of APP by BACE1 releases a soluble extracellular fragment and is followed by APP cleavage within its transmembrane domain by g-secretase.
The second cleavage releases the intracellular domain of APP and b-amyloid.
The immature form of BACE1 contains an N-terminal signal sequence (residues 1–21) followed by a large catalytic domain, a single transmembrane domain (residues 461–477), and a short cytoplasmic domain (residues 478–501).
The signal sequence (1-21) is cleaved from the immature form, yielding the proBACE1 protein, which starts at residue 22.
The proBACE1 protein is modified by cleavage of 12 N-terminal residues (aa 22-45), producing the mature BACE1 protein.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.05-0.2ug/mlWestern Blot: 0.1-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：40ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 30% glycerol.
Specificity：Recognizes human BACE1. Species sequence homology: mouse, rat and bovine
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa485-50, CLRQQHDDFADDISLLK at C-terminal of human BACE1.
