AP2M1 is a subunit of heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complex medium subunit family.
AP2 is composed of two large adaptins (alpha-type subunit AP2A1, or AP2A2, and beta-type subunit AP2B1), one medium adaptin (mu-type subunit AP2M1), and a small adaptin (sigma-type subunit AP2S1).
The AP2 complex is part of the protein coat linking clathrin to receptors in coated vesicles.
Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins so that they can be selected and concentrated.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.05-0.2ug/mlWestern Blot: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:40ug
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide.
Specificity:Recognizes human AP2M1 protein.
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide . within residues 150-200 of human AP2M1 protein (KLH) (Swiss Prot: Q96CW1).