AMPK alpha-1 chain (5'-AMP-activated protein kinase catalytic subunit alpha-1) is responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase.
It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase.
It appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia.
It is expressed in kidney, liver, lung, heart, and brain tissues.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.05-0.2ug/mlWestern Blot: 1.0ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：40ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 30% glycerol.
Specificity：Recognizes mouse AMPK alpha-1 chain.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide TSPPDSFLDDHHLTR conjugated to KLH
