Akt, also known as protein kinase B (PKB), a serine/ threonine kinase, is a critical enzyme in several signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes.
Akt is activated following its phosphorylation at two regulatory residues.
Phosphorylation of threonine on the kinase domain, catalyzed by PDK1, is essential for Akt activation.
Akt activity is augmented approximately ten fold by phosphorylation at the serine on the hydrophobic motif by PDK2.
Phosphorylation of Thr308 and Ser473 activates Akt alpha.
Phosphorylation at Thr309 and Ser474 on Akt beta1 and beta2, and on Thr305 on Akt gamma results in their activation.
Akt promotes cell survival by inhibiting apoptosis by phosphorylating and inactivating several targets, including bad, forkhead transcription factors, c-Raf and caspase-9.
The activation of Akt is negatively regulated by PTEN, a PIP3-specific phosphatase, and SHIP, a SH2-domain containing inositol 5-phosphatase.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.05-0.2ug/mlWestern Blot: 0.5-2.0ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：40ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 30% glycerol, 0.02% sodium azide.
Specificity：Recognizes endogenous human Akt1, Akt2 and Akt3, phosphorylated at serine 473, serine 474 and serine 472, respectively. Species Crossreactivity: mouse, rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to human Akt from residues surrounding the phosphorylation site of Serine 473 (Q-F-SP-Y-S). This sequence is identical to human, rat and mouse Akt2 and Akt3.
