Roundabouts (ROBO) are cell-surface receptors that mediate repulsive signaling mechanisms at the central nervous system midline.
However, ROBOs may also mediate attraction mechanisms in the context of vascular development.
ROBO4 is a novel roundabout protein which is restricted in expression to endothelial cells in vitro and sites of angiogenesis in vivo.
Because it is not expressed on normal endothelial cells, in vivo, it is a promising tumor endothelial marker.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Positive Control: HUVEC lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris-citrate/phosphate, pH 7-8, 0.09% sodium azide.
Specificity：Recognizes the 85kD and 107kD isoforms of the ROBO4 human protein.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to an internal region of the human protein (within residues 200-300). Species sequence homology: 100% homology with all 4 isoforms of the human protein. There is no homology with mouse or rat proteins.
